Lab. No

1. Orientation to microbiology laboratory.

    a. Safty procedure and precaution.

    b. General laboratory directions

    c. The microscope.

2- Mixed Culture and preparation

   a. Simple stain.

    b. Gram's stain method.

3- Culture Media

    a. Preparation of nutrient agar plate.

    b. Culturing microorganisms from the environment.

4- Examination of the culture of the previous laboratory from different colonies

Sterilization and Disinfection

5- Pure Culture Technique

6- Study colonial morphology

Sub-culture technique

7- Study of some metabolic and enzyme activities of bacteria.

8- Immunology:

   a. Precipitation (ring test, Single and double immunodifution, immunoelectrophoresis)

   b. Agglutination: (slide agglutination, test tube agglutination)

   c. Complement fixation.

  d. E-Rosette, EA-Rosette, Prick test, Mononuclear cell isolation.

9- Systemic Bacteriology

10- Staphylococci:

    a. Inoculate and streak blood agar plate and mannitol salt agar with the culture provided.

    b. Make Gram stain of the organism.

11- Streptococci:

    a. Describe the appearance of staphylococci on Blood agar and manitol salt agar plates.

    b. Perform a slide coagulase test on each of the staphylococcus agar plate.

    c. Inoculate and streak blood agar plate with the culture provided.

   d. Make Gram' stain of the organism.

12- Pneumococci:

    a. Describe the appearance of streptococci on Blood agar.

     b. Subculture streptococci in tube of brain heart infusion broth.

    c. Inoculate and streak blood agar plate with the culture provided.

    d. Make Gram' stain of the organism.

13- Neisseriae:

    a. Prepare Gram stained slide from the Brain-heart infusion broth. Describe the microscopic morphology of streptococci in liquid medium.

    b. Describe the appearance of Pneumococci on Blood agar.

     c. Subculture Pneumococci in brain-heart infusion broth and incubate at 37'c

    d. Inoculate and streak blood agar plate with N. Catarralis.

    e. Make Gram' stain of N. Catarralis.

    f. Stain the prepared slide provided with Gram method

14- Corynebacteria:

    a. Stain the prepared slide provided with Neisser's stain.

    b. Perform the bile solubility test for pneumococcus.

    c. Perform the oxides test of N. Catarrhalis.

15- Mycobacteria

Stain the slide of sputum with Zeil-Nelsen stain.

16- Genus Bacillus:

   a. Inoculate and streak blood agar plate with Bacillus subills.

   b. Make Gram' stain slide of the organism.

   c. Prepare heat fixed smear of the culture and stain it with spore stain.

17- Genus Clostridia:

  a. Describe the appearance of B. Subtilis on blood agar.

   b. Demonstration on clostridia.

   c. Film on Gas Gangrene

Enteric Bacilli:

18- Echerichia coli and klebsiella aerogenes.

   A. Inoculate and streak macconkey agar plate with the culture provided.

   b. Make Gram' stain of the organism.

19- Proteus and Pseudomonas

   a. Describe the appearance of E. coli and K. aerogenes on Macconkey agar plate.

   b. Subculture the organism on the following media:

     - Peptone water.

     - Glucose broth (two tubes).

    - Slant of Simmon Citrate agar.

   c. Inoculate and streak Macconkey agar plate with the culture provided (Proteus and Pseudomonas).

   d. Make Gram' stain of the organism.

20- Vibrios

   A. Demonstration on Non-aggulatinable vibrios (NAG strain).

    b. Describe the appearance of Pseudomonas on Macconkey agar plate.

    c. Perform the oxidase test on pseudomonas culture;

    d. Examine the glucose broth incubated with proteus.

    e. Examine the urea broth incubated with proteus.

21- Spirochetes, Hemoophiliusand Bordetelia:

   a. Slide demonstration on Trepanema palladium, hemophillis ducrevil and Borrelia vincenti.

   b. Examine pseudomonas agar slant for pigment production.

   c. Examine the glucose sugar broth (fermentation).

Virology

22- First lab.

a. Isolation of the viruses:

- Tissue culture

- Embryonated agg.

- Animal inoculation.

b. Histological examination.

c. Transformation.

d. Slide projection

23- Second lab:

a. Serological tests for identification of viruses:

- Viral neutralization.

- Haemagglutination & haemagglutination inhibition

- Plague and plague reduction.

- Gel diffusion.

- ELISA

- Complement fixation test.

- Slide Projection.

Mycology

24- First lab

  a. Contaminants.

   b. Dematophytes.

   c. Candida species (yeast-like fungi).

25- Second lab: (systemic fungi)

   a. Cryptococcus.

   b. Histoplasma capsulatum.

   c. Sporotrichum schenkii.

26- Final Examination.